Monday, June 27, 2011

About Hybrid Seeds

This article I would like to share my knowledge about Hybrid seeds from my lecturer during my study in Plant Breeding Topics in University. Firstly he said that Malaysia is in early stage of producing our own hybrid seeds for selected crops. In the opening speech he said that the history of Hybrid Seeds began with maize started in the 1920s and it became extended to vegetables and flowers and more recently in rice and some forage crops. He said in the later part he will touch about Genetically Modified Seeds. This lecture is related to my final year thesis paper as part in the masters basic requirements. I hope this article able to enhance our knowledge about Hybrid Seeds and their contribution to global food supply.

Hybrid seeds are produced from naturally out-breeding crops or from which inbred lines are produced by repeated self-pollination. The established inbred lines are crossed to produce First generations (F1) hybrid seeds. The hybrid seeds are prized because they produce uniform plants benefiting from the effect called heterosis (hybrid vigor). Heterosis can result in a large increase in yield over the inbred lines or comparable lines that are out-crossing. The precise basis of heterosis is still unclear, but epistasis and over-dominance are thought to be involved. Epistasis is the interaction between different genes and over-dominance is a condition where the heterozygotes (genes represented by two different versions) are superior to either homozygotes (gene represented by the same versions). The F1 hybrid seed is heterozygous in many genes.

The purpose of Hybrid seed is planted to produce a crop that is harvested for use. Saving seed from the crop and planting it is undesirable because the two different versions of the genes in the F1 hybrid segregate out in the offspring and producing an extremely variable progeny. In other words he said the superior qualities of the F1 hybrid will have all disappeared. The hybrid is obtained by crossing the inbred lines in which therefore, have to be separately maintained. Thus, only the seed companies produce hybrid seeds and farmers must buy those seeds from the company every year. Malaysia import huge amount of hybrid seed amounting more than RM 15 million annually. The hybrid seeds are leafy vegetable seeds, watermelon seeds, Fruity vegetable seeds and melon seeds and of course sweet corn.

According to my lecturer in university he did mentions that scientists by the name of Dr Wallace graduated from University with an agriculture degree and later studied statistics thereafter on his own. He later taught the subject at Iowa State University and used his knowledge to develop the first commercial hybrid maize. In 1926, he founded the Hi-Bred Corn company (now Pioneer Hi-Bred Seed Company, a subsidiary of Dupont Chemical Company) and later entered politics. He was made Secretary of Agriculture before being elected vice-president of the United States. Wallace was noted for his concern for the common man and envisioned hybrid corn as a means of providing bountiful food at low prices for the masses.

According to my lecturer, the first corn hybrids were made by detasseling the plants of the maternal inbred-line by removing the male flowers so that the female flowers on the plants can only be fertilized by pollen produced from plants of another male line. The detasseling operation used to be performed mainly by young girls employed during the summer months. Later on the male-sterile lines were developed that did not produce fertile male flowers or pollen. The male-sterile maternal lines were fertilized with paternal lines that allowed the hybrid seed to produce both male and female flowers. The male-sterile lines corn are most frequently altered in the mitochondrial genome which leading to the inhibition of male flower development . A number of such lines are now available.

He said that the early development of male-sterile lines led to disaster, however. The primary line used in the 1960s contained the T (Texas) cytoplasm male-sterility gene and by 1970, over 85% of the commercial maize planted contained that gene. The gene also caused a pleiotropic (multiple effects due to a single gene) susceptibility to a fungus disease. During a the 1970 summer the disease spread widely particularly in the summer corn belt disease. The impact on maize production was disastrous and leading to a return to hand-detasseling for a number of years until alternate male-sterility lines could be developed. The lesson that should have been learned was that the absence of diversity is bound to lead to disastrous epidemics but that lesson tends to get ignored in favour of risky but profitable genetic manipulations. The history is an important record for plant breeders to remember.

My lecturer continues to say that Rice Hybrids have been produced using cytoplasmic male-sterility. Over-dominance and epistatic genes were implicated as the basis for heterosis (and inbreeding depression, a phenomenon in which inbred lines suffer decreased yield). Alfalfa interspecies hybrids showed heterosis where the interspecies hybrids are a little different from those originating from inbred lines but in general they act similarly to inbred lines. At this time I really confused about the facts given by my lecturer. I have to read more related reference books in the library to go deeps in this topic.

He continues his lecture by saying that a large number of vegetable crops have been hybridized. Hybrid cucumbers have been produced by hand pollination by the removal of male flowers or  their gynoecy (property of producing only female flowers). There does not seem to be an available male sterility gene . Hot and sweet peppers have been hybridized. Both nuclear and cytoplasmic sterility are used in some cases. Most hybrid-pepper seed production is carried out in China, India or Thailand but not in Malaysia. About two-thirds of commercial onions are hybrids. Malaysia does not produce onions but import more than USD 3.45 million annually. These are produced using male sterility lines. Hybrid cabbage shows strong heterosis and the use of such hybrids is expanding. The seed is produced using male sterile lines.

Since most of the male sterile lines used commercially contain mitochondrial genes but such genes are not readily available in a number of crops. Genetic engineers have developed a system of male-sterility based on transformation of the chloroplast with a gene for beta-ketothiolase that interferes with fatty acid synthesis that leading to disrupted anther tissue and a failure to produce pollen. The beta-ketothiolase gene is controlled by a light sensitive promoter and so that male-fertility can be restored in hybrids using several days of continual illumination. The system was developed in tobacco but may be extended to food crops, barring unforeseen complications. At this time I almost very sleepy….

When my Lecturer start to move to a new topic that’s is GM.. I awake with round eyes. He said a number of Genetically Modified (GM) male-sterile crops have been developed and tested in the field. In Canada a male-sterile transgene was introduced into the nuclear gnome of canola and then that construction was approved for and has been in commercial production. The transgenic construct included a barnase ribonuclease gene controlled by a tapetum promoter. Barnase kills pollen cells thus rendering the plant male-sterile. In the hybrid male fertility is restored using the barstar inhibitor of barnase although barnase is well known to be toxic to animal cells. The development continued and the technology came to be used to protect GM traits patented by agribusiness corporations such as herbicide tolerance under the general rubric of genetic use restriction technology. Such crops were extensively field tested in Europe and we have warned that the F1 hybrid grown in the field will actually spread the barnase transgene as well as the herbicide tolerance gene in pollen with potentially harmful ecological impacts. The toxin may well be carried over into the canola press cake used both for both food and feed.

His lecture continue with a tired sound that the development of hybrid seed had left seed production to seed companies for the practical reason that it is the most economical way to maintain appropriate inbred lines and seed production can be isolated from the food production areas of open pollinating crops. But it had also prevented farmers from saving and replanting seeds and making it necessary to purchase seeds every season.

He said in future the biotechnology has gone a step further and demanded that seed production be restricted to companies even when there is no rational basis for the restriction other than corporate greed. They argued that developed countries could benefit from the additional production supposedly to be gained by the technology, while developing countries will suffer from their inability to afford the high extra cost. They predict net deterioration in the developing countries due to the widening gap in productivity. These predictions must be taken with a very large grain of salt. There is at present no evidence that genetic use restriction technologies, or indeed, any genetic modification technology have led to increase in crop yield.

He later said that both hybrids and GM crops lack the diversity required for sustainability in the complex ecosystems of the developing world. What is needed is seed production that takes into account the unique requirements of developing countries, where the farmers’ rights to save, replant and exchange seeds are integral to food sovereignty and food security. As he completed his lecture I am full with the new knowledge. It was a long 2 hours lecture in the cool room and I hope I am able to get a high mark in the exam later. That’s all folks for this entry…

Related articles:
1. OP vs Hybrid seeds (Click here)
2. Pollination (Click here)
3. Benih Sayuran (Klok disini)
4. Harga Benih Sayuran (Klik disini)
5. Industri Benih Malaysia (Klik disini)
6. Kontrol Benih Dunia (Klik disini)
7. Known You Seeds , Taiwan (Klik disini)

M Anem
(Monday 7.30 am)

No comments:

Post a Comment

Note: Only a member of this blog may post a comment.


Related Posts Plugin for WordPress, Blogger...